| 质粒名称: | pPhb2-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000753 |
| 产品用途: | Expresses phb2-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | prohibitin2, mScarlet3-H |
| 物种来源: |
H.sapiens, Synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pPhb2-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pPhb2-mScarlet3-H (aka mYongHong)载体质粒基本信息
pPhb2-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pPhb2-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pPhb2-mScarlet3-H (aka mYongHong)质粒载体简介
pPhb2-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pPhb2-mScarlet3-H (aka mYongHong) 4824 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_753_20241014-BAN4_D03_D03_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4824)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4824
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 424..727
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 728..931
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 960..1109
/codon_start=1
/label=Prohibitin-2
/translation="MAQNLKDLAGRLPAGPRGMGTALKLLLGAGAVAYGVRESVFTVEG
GHRAI"
regulatory 1155..1164
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1161..1847
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
polyA_signal 1975..2096
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2103..2558)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2585..2689
/gene="bla"
/label=AmpR promoter
promoter 2691..3048
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2899..3034
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3083..3877
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4109..4156
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin join(4485..4824,1..249)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
61 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
361 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
421 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
481 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
541 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
601 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
661 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
721 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
781 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
841 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
901 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagca
961 tggcccagaa cttgaaggac ttggcgggac ggctgcccgc cgggccccgg ggcatgggca
1021 cggccctgaa gctgttgctg ggggccggcg ccgtggccta cggtgtgcgc gaatctgtgt
1081 tcaccgtgga aggcgggcac agagccatcg gaggaagtgg aggcagggta ccgcgggccc
1141 gggatccacc ggtcgccacc atggatagca ccgaggcagt gatcaaggag ttcatgcggt
1201 tcaaggtgca catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
1261 agggccgccc ctacgagggc acccagaccg ccaagctgag ggtgaccaag ggtggccccc
1321 tgcccttctc ctgggacatc ctgtcccctc agttcatgta cggctccagg gccttcacga
1381 agcaccccgc cgacatcccc gactactgga agcagtcctt ccccgagggc ttcaagtggg
1441 agcgcgtgat gaacttcgag gacggcggcg ccgtgtccgt ggcccaggac acctccctgg
1501 aggacggcac cctgatctac aaggtgaagc tccgcggcac caacttccct cctgacggcc
1561 ccgtaatgca gaagaagaca atgggctggg aagcatccac cgagcggttg taccccgagg
1621 acgtcgtgct gaagggcgac attaagcatg ccctgcgcct gaaggacggc ggccggtacc
1681 tggcggactt caagaccacc tacagggcca agaagcccgt gcagatgccc ggcgccttca
1741 acatcgaccg caagttggac atcacatccc acaacgagga ctacaccgtg gtggaacagt
1801 acgaacgctc cgtggcccgc cactccaccg gcggctccgg tggctcctaa taagcggccg
1861 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
1921 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
1981 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
2041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
2101 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
2161 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
2221 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
2281 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
2341 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
2401 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
2461 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
2521 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
2581 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
2641 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
2701 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
2761 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
2821 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
2881 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
2941 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
3001 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
3061 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
3121 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
3181 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
3241 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
3301 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
3361 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
3421 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
3481 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
3541 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
3601 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
3661 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
3721 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
3781 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
3841 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
3901 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
3961 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
4021 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
4081 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
4141 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
4201 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
4261 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
4321 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
4381 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
4441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
4501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
4561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
4621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
4681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
4741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
4801 tgaacggggg gttcgtgcac acag
//
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pPhb2-mScarlet3-H (aka mYongHong)质粒载体应用举例
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